Solubilization and Purification of the Gonadotropin (GTH II) Receptor from Rainbow Trout (Oncorhynchus mykiss) Ovaries
نویسندگان
چکیده
منابع مشابه
Rainbow Trout, Oncorhynchus mykiss
Rainbow trout are classified as Oncorhynchus mykiss, and as such belong to the same genus as Pacific salmon, and to the family Salmonidae, which includes Atlantic salmon (Salmo salar), various trout (Salvelinus sp.), Arctic char (Salvelinus alpinus), Arctic grayling (Thymallus arcticus) and whitefish (Coregonus sp.). Rainbow trout are native to areas around the North Pacific Ocean, from souther...
متن کاملPartial Purification and Characterization of Rhodanese from Rainbow Trout (Oncorhynchus mykiss) Liver
Cyanide is one of the most toxic substances present in a wide variety of food materials that are consumed by animals. Rhodanese, a ubiquitous enzyme, can catalyse the detoxification of cyanide by sulphuration reaction. In this study, rhodanese was partially purified and characterized from the liver tissue homogenate of the rainbow trout. The enzyme was active in a broad range of pH, from 5 to 1...
متن کاملPost-ovulatory secretion of pituitary gonadotropins GtH I and GtH II in the rainbow trout (Oncorhynchus mykiss): regulation by steroids and possible role of non-steroidal gonadal factors.
In order to determine the factors of ovarian origin which can modulate the postovulatory secretion of the FSH-like gonadotropin (GtH I) and the LH-like gonadotropin (GtH II), freshly ovulated female rainbow trout were divided into two groups. In the first group the fish were stripped in order to eliminate the eggs and ovarian fluid from the body cavity, while in the second group the eggs were k...
متن کاملValidation of radioimmunoassays for two salmon gonadotropins (GTH I and GTH II) and their plasma concentrations throughout the reproductivecycle in male and female rainbow trout (Oncorhynchus mykiss).
RIAs were developed for the two salmon gonadotropins (GTH I and GTH II) and used to measure plasma GTH throughout the life of the rainbow trout, Oncorhynchus mykiss. The RIA for GTH II was specific and sensitive (< 0.001% cross-reaction with GTH I, mean sensitivity = 0.26 +/- 0.02 ng/ml). The RIA for GTH I was less specific and less sensitive than the GTH II RIA (9.7% cross-reaction with GTH II...
متن کاملMetabolism in Erythrocytes of the Rainbow Trout Oncorhynchus Mykiss
Experiments were carried out to investigate the relationship between ion exchange and energy metabolism in rainbow trout erythrocytes in vitro. Under resting conditions, the sodium/potassium pump accounts for 20 % of the cellular energy budget. In the presence of the /S-adrenergic agonist isoproterenol, however, this increases to 43 %. Inhibition of the sodium/potassium pump with ouabain result...
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ژورنال
عنوان ژورنال: General and Comparative Endocrinology
سال: 1993
ISSN: 0016-6480
DOI: 10.1006/gcen.1993.1127